Cyclic Immunofluorescence (CycIF) & Spatial Biology Services

High-plex multiplex immunofluorescence and spatial biology workflows for tumor microenvironment analysis, immune profiling, biomarker discovery, and translational research powered by Lunaphore COMET™ technology.

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Lunaphore COMET™ Multiplex Tissue Imaging

Automated cyclic immunofluorescence workflows for scalable high-dimensional tissue imaging and spatial biomarker analysis.

What is Cyclic Immunofluorescence (CyCIF)?

CyCIF enables repeated cycles of antibody staining and fluorescence imaging to visualize dozens of protein biomarkers within intact tissue architecture while preserving spatial context and cellular organization.

Multiplex Immunofluorescence & Spatial Biology Services:

IMCO provides end-to-end CyCIF services including experimental design consultation, antibody panel optimization, tissue imaging, spatial analysis, and downstream bioinformatics support.

Why we use COMET cycIF in IMCO labs:

  • Ultra-high plexing: simultaneous visualization of 30+ markers in a single tissue section with precise spatial resolution.

  • Same slide H&E for AI driven data analysis.

  • Spatial biology, uncoupled from single-marker limitations: understand cell–cell interactions, neighborhoods, and microenvironment patterns.

  • FFPE-compatible workflows: work with routinely collected clinical samples without complex preprocessing.

  • Rapid, automated workflow: streamlined protocols reduce hands-on time and improve consistency.

  • Quantitative, reproducible data: standardized cycles enable robust image analysis and cross-sample comparability.

  • End-to-end readiness: integrates with our imaging platforms and data analytics to accelerate discovery and decision-making. Learn more about the Lunaphore Comet here.

Illustration of interconnected blue shapes with one orange circle in the bottom right corner.

Featured Panels

A blue slide displaying a list of 13 names of core biological markers or proteins, including a-SMA, CD11c, Foxp3, CCR2, CD20, GRZB, CD3, CD45, Ki-67, CD8, CD68, and PANCK.

Core Panel

Targets: CD8 T Cells, Tregs, Th Cells, B Cells, Monocytes, Macrophages, DCs, Neoplastic Cells, Fibroblast-like Cells

Function: Proliferation, Cytotoxicity

An orange discovery panel listing 20 items related to cellular biology, including markers like a-SMA, CD8, CD45, CD163, Ki-67, CCR2, CD11b, CD68, Foxp3, PANCK, CD3, CD11c, CD69, GRZB, PD-1, CD4, CD20, CD103, HLA-II, PD-L1.

Discovery Panel

Targets: CD8 T Cells, Tregs, Th Cells, Unconventional T Cells, B Cells, Monocytes, Macrophages, DCs, Other APCs, Neoplastic Cells, Fibroblast-like Cells

Function: Proliferation, Activation, Checkpoint, Cytotoxicity, Tissue Residency, Anti-inflammatory(M2-like)

Green chart titled "Discovery T Function (29)" listing various cell markers and proteins such as CD11c, CD68, GRZB, PD-L1, with rows of other markers and proteins including CD3, CD4, CD8, and CD45.

Discovery T-Function

Targets: CD8 T Cells, T-regs, Th1 Cells, Th2 Cells, Unconventional Cells, B Cells, Monocytes, Macrophages, DCs, Other APCs, Neoplastic Cells, Fibroblast-like Cells

Function: Proliferation, Activation, Cytotoxicity, Tissue Residency, Memory, Exhaustion, Self-renewal, Immunosuppression, Anti-inflammatory (M2-like)

Custom Panels

Chart titled 'AML Bone Marrow (19)' listing various cell markers, including a-SMA, CD11c, CD45RA, CD163, CD3, CD20, CD61, Foxp3, CD4, CD31, CD68, Ki-67, CD8, CD34, CD71, MPO, CD11b, CD45, CD117, with some markers highlighted in orange.
A table listing 16 different cell markers related to diffuse large B-cell lymphoma (DLBCL), including CD3, CD20, EOMES, LAG-3, CD4, CD45, FOXP3, PD-1, CD8, CD45RA, GRZB, TBET, CD19, CD69, Ki-67, and TIM-3.
Table showing neural cell markers, with some in white and others in orange, titled 'Neuronal (13)' on a light blue background.
A presentation slide titled "Carcinoma (20)" listing various immunohistochemical markers such as a-SMA, CD11b, CD69, HLA-II, CCR2, CD11c, CD103, Ki-67, CD3, CD20, CD163, PANCK, CD4, CD45, Foxp3, PD-1, CD8, CD68, GRZB, PD-L1.
A table titled 'Melanoma (17)' listing various biomarkers and genes associated with melanoma, including Melanosome and SOX-10 in orange text.
Table titled 'Dermatofibrosarcoma Protuberans (DFSP) (32)'. It lists various cell surface markers, with some listed in white, and CD21 and CD23 in orange. The markers include a-SMA, CD20, CD68, GATA-3, PD-1, CCR2, CD69, GRZB, PD-L1, CD3, CD103, HLA-II, TBET, CD4, CD39, CD163, Ki-67, TCF1, CD8, CD45, DC-LAMP, LAG-3, TIM-3, CD11b, CD45RA, Foxp3, PANCK, TOX, CD11c, CD45RO.
A chart titled 'Melanoma - Fresh Frozen (33)' listing various genetic markers and proteins associated with melanoma, including CD20, CD39, PD-1, and BRAF, with some in orange and others in blue.
Microscopic view of green, circular cells with blue nuclei and a single purple-stained cell among them.

Breast Cancer: Pan-CK, CD45

Microscopic view of cells with green and yellow fluorescent markings against a blue background.

Melanoma TLS: CD8, ASMA

Microscopic view of a biological tissue sample with green, purple, blue, and orange colored structures.

Lung: CD4, CD8, ASMA, Pan-CK

Fluorescent microscopy image showing two groups of cells, one with green fluorescent labels on the left and one with red and blue fluorescent labels on the right, against a black background.

Sarcoma: CD3, CD8, Pan-CK

A digital, abstract visualization of a face with blue and orange points and lines on a black background.

Melanoma: DAPI, POSTN

Microscopic view of cells with turquoise, orange, and purple fluorescent staining on a black background.

Melanoma TLS: Ki-67