Mass Cytometry by Time-of-Flight (CyTOF)
Mass cytometry by time-of-flight, or CyTOF, is a single-cell proteomics technology capable of interrogation of more than 40 markers simultaneously in a liquid suspension. This technology empowers researchers to look deeper, unveiling new cell types and their functions.
This high-resolution approach allows for prospective and retrospective, highly standardized and reproducible analysis of clinical samples (peripheral blood or tissue suspension):
Immune cell activation and inhibition state
Intra- and interpatient analyses
Predictive biomarkers for treatment stratification
Identification of response vs resistance to treatment
On treatment changes reflective of therapeutic engagement
Methodology:
Helios™, a high-performance mass cytometer, is a state of-the-art instrument representing the greatest advances in mass cytometry and is designed to provide a new tool for bioanalytical single-cell detection and analysis. The Helios system analyzes individual cells labeled with stable heavy metal isotopes with negligible overlap which allows for unparalleled ability to generate high-resolution phenotypic and functional profiles of cells from normal and diseased states. Our CyTOF panels utilize the MaxPar Direct Assay which is designed and optimized for deep profiling of human blood. The use of the core assay with customizable additions is easily scalable, highly reproducible and validated across multiple patient cohorts and disease states.
Available panels:
MaxPar: 30 markers allow for single cell resolution of 37 immunophenotypes
MaxPar Plus: custom panel incorporates functional markers (activation, proliferation, check points) on the MaxPar backbone.
*CUSTOM PANELS and VALIDATION AVAILABLE
Specimen Requirements:
The CyTOF analysis is compatible with whole blood samples collected in NaHep tubes or processed frozen PBMCs (peripheral blood mononuclear cells).
Data visualization and interpretation examples
1. Immunophenotyping Analysis
